Workshop and Symposium on CRISPR/Cas9
April 24th to 26th, 2018
Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo (FMRP- USP)
Workshop on CRISPR/Cas9
April 24th, Tuesday
08:30 - Registration and material distribution (light coffee provided)
9:00 - Welcome and Introductory remarks (Lucila Elias and Thiago Cunha)
9:15 - 10:15 Principles of Gene Targeting and Genome Editing – Thom Saunders
10:15 to 10:30 - Coffee Break
10:30 to 12:00 - Overview of CRISPR-Cas9 – Thom Saunders
12:00 to 14:00 - Lunch
14:00 to 18:00 - Seminar: Selected papers to be discussed (defined in advance by Dr. Saunders)
April 25th, Wednesday
08:30 to 12:00 - CRISPR-Cas9: choosing your genes, design, cloning (Part I) – Hands on (students should bring laptops) - Thom Saunders
14:00 to 17:00 - CRISPR-Cas9: testing, pitfalls (Part II) - Hands on (students should bring laptops) - Thom Saunders
Symposium on CRISPR/Cas9
April 26th, Thursday
8:00 – 8:20 Registration and material distribution
8:20 – 8:30 Welcome Greetings Carol Elias and Lucila Elias
8:30 – 9:20 Conference - Chairs: Lucila Elias and Susan Allen (University of Michigan)
Carol Elias and Thom Saunders (University of Michigan, USA) – A journey into the mysteries and delights of mouse genetics
9:20 – 10:40 Symposium # 1 ‐ Chairs: Thiago Mattar Cunha (FMRP-USP) and Marcio Torsoni (UNICAMP)
Angela Saito (LNBio- Campinas) - Creating knockout and knockin mouse models using CRISPR/Cas9 system
Clarissa Ribeiro (ICB-USP) ‐ Title - Revealing novel temozolomide resistance mechanisms via CRISPR genomic screening
Short Talk
Fernando Simabuco (UNICAMP) - Applications of CRISPR Cas9 mediated genome editing in cell culture models
10:40 – 11:00 Coffee Break
11:00 – 12:20 Symposium # 2 ‐ Chairs: José Carlos Alves Filho (FMRP-USP) and Carol Elias (University of Michigan)
Katiuchia Salles (FMRP-USP) - CRISPR as a tool to investigate serine proteases in cancer
Elisa Prestes (UFRJ) - CRISPR: a new tool for studying oxidative stress
Short Talk
Gabriel Publio (FMRP-USP) - Generation of PKM2 knockout HaCat cell line by CRISPR/Cas-9 mediated genome editing
12:20 – 14:30 Lunch
14:30 – 16:10 Symposium # 3 Chair: Beatriz Borges (FMRP-USP)
Geraldo A. Passos (USP Ribeirão Preto) - Crispr-Cas9 as a tool for gene disruption in thymus gland cells
Tiago Campos Pereira (FFCLRP-USP) - Identification of anhydrobiosis-related genes using CRISPR
Short Talk
Tatiana Nerry Cecilio (Oxford-UK and FMRP-USP) - A genome-wide CRISPR screen in primary immune cells: challenges and perspectives
Gabriel Gonçalves (FMRP-USP) - Adrenal Carcinoma Modeling with CRISPR-Cas9
16:10 – 16:30 Coffee Break
16:30 – 17:30 Round Table # Concluding Remarks and Perspectives Thom Saunders, Carol Elias (Univ of Michigan), Beatriz Borges, Lucila Elias,
Thiago Cunha and Katiuchia Salles (FMRP-USP), Márcio Torsoni (UNICAMP).
